Experiments Show Infection of Visceral Fat Cells May Contribute to Severe COVID-19

Cultured differentiated adipocytes: lipids are stained purple; the protein ACE-2, which serves because the receptor for SARS-CoV-2, is inexperienced; cell nuclei are blue. Credit score: Amanda Passos & Flávio Protasio Veras

Two kinds of adipocytes (fats cells) had been contaminated within the laboratory: one obtained from human stem cells remoted from subcutaneous tissue and the opposite differentiated from stem cells taken from visceral fatty tissue.

Experiments present that visceral fats – fats across the liver, intestines, and different organs, thought-about a threat issue for heart problems, diabetes, and hypertension – contributes extra to extreme COVID-19 than subcutaneous fat (under the skin, as in “love handles”). The experiments were conducted in Brazil by researchers at the State University of Campinas (UNICAMP) and the University of São Paulo (USP).

In order to arrive at this conclusion, Marcelo Mori, a professor at the Institute of Biology at UNICAMP and one of the study’s leaders, infected in the lab two different types of fat cells: one obtained from human stem cells isolated from subcutaneous tissue and the other differentiated from stem cells taken from visceral fatty tissue.

“It was possible to observe that visceral adipocytes are more susceptible to infection by SARS-CoV-2. Viral load increased far more in this fat cell type than in subcutaneous adipocytes. We believe this was due mainly to higher levels of the protein ACE-2 [to which the virus binds to invade cells] on the cell floor,” Mori advised Agência FAPESP.

Moreover, the researchers found that when visceral adipocytes had been contaminated, they produced a bigger quantity of pro-inflammatory cytokines, which alert the immune system to the existence of a risk to be combated.

An article on the examine was printed just lately within the scientific journal Nature Communications. Several research groups at UNICAMP collaborated with the group at USP, alongside colleagues at the Brazilian Bioscience National Laboratory (LNBio-CNPEM), National Cancer Institute (INCA) and D’Or Research and Education Institute (IDOR). The principal investigators were Luiz O. Leiria (USP), Mariana Osako (USP) and Daniel Martins-de-Souza (UNICAMP). The study was funded by FAPESP via 20 projects (17/08264-8, 20/05040-4, 20/04746-0, 19/00098-7, 19/05155-9, 20/04583-4, 20/15959-5, 19/26119-0, 16/00194-8, 20/04558-0, 20/04579-7, 21/10373-5, 20/08716-9, 13/07607-8, 20/04919-2, 17/01184-9, 17/23920-9, 16/24163-4, 19/04726-2 and 18/21635-8).

Viral reservoir

Mori’s team at UNICAMP was the first in the world to show – in July 2020 – that SARS-CoV-2 can infect human fat cells and to suggest that adipose tissue serves as a reservoir for the virus.

“After that, other studies confirmed that adipocytes can indeed be infected, and when we analyzed samples from patients who died of COVID-19, we found the presence of the virus in adipose tissue to be relatively frequent, corresponding to about 50% of cases,” Mori said.

The group then decided to investigate whether there were differences between the way visceral and subcutaneous adipose cells responded to infection. As far as metabolic diseases are concerned, the evidence in the scientific literature shows visceral fat to be the main villain, while subcutaneous fat tends to be neutral or even beneficial.

“We wanted to see if there was a similar association in the context of COVID-19,” Mori said. “And in fact, our model suggests that the more visceral adipose tissue there is in obese individuals, the more the virus can replicate, and this amplifies the inflammatory process.”

In subcutaneous adipocytes, on the other hand, the group observed a decrease in lipolysis, the breakdown of fats and other lipids by hydrolysis to release fatty acids, which can be used as a source of energy during physical activity or fasting periods.

“Our hypothesis is that this represents an antiviral cellular response,” Mori said. “There are studies showing that inhibition of lipolysis lowers the replicative capacity of SARS-CoV-2, which can be explained by the fact that the virus needs lipids to produce its envelope, as well as energy from cells to make copies of its genetic material.” Reduced lipolysis in subcutaneous adipose tissue, therefore, could be positive for the patient and bad news for the virus.

Contrasting responses

Visceral adipocytes were exposed to different strains of SARS-CoV-2: the ancestral lineage originally from Wuhan, China, and isolated from one of the first Brazilians diagnosed with COVID-19; and the gamma variant (P.1.), which emerged in late 2020 in Manaus, the capital of Amazonas state in Brazil. The difference in susceptibility compared to subcutaneous adipocytes was observed only in response to the ancestral virus.

“We concluded that the Manaus variant is less effective at infecting visceral fat cells than the ancestral strain,” Mori said. “A proteomic analysis [of all the proteins produced by the cells] confirmed that the Wuhan pressure led to a discount in a number of proteins related to the mobile response to interferon [ an immune system mechanism to combat viruses], whereas the gamma variant led to a rise. In different phrases, the Manaus pressure made adipocytes produce extra proteins that promote an antiviral response.”

Current analysis factors to a downtrend within the variety of extreme instances of COVID-19 as a consequence of novel variants amongst folks with weight problems. “However this may very well be influenced by different elements, akin to vaccination or prior an infection. Or these people could have been taking further care as a result of they knew they belonged to a high-risk group,” Mori defined.

To attempt to deepen their understanding of all these processes, the group plans additional experiments involving adipocytes cultured with the delta and omicron variants.

One other plan for future analysis is to analyze potential medium- to long-term metabolic impacts of an infection by SARS-CoV-2. “We wish to discover out whether or not an infection adjustments the chance of growing diabetes or heart problems, for instance,” Mori stated. “A technique of doing so may very well be to research samples from sufferers who had COVID-19 and had been later submitted to bariatric surgical procedure, in an effort to see if morphological and useful alterations occurred in visceral adipose tissue on account of the an infection.”

Reference: “SARS-CoV-2 infects adipose tissue in a fats depot- and viral lineage-dependent method” by Tatiana Dandolini Saccon, Felippe Mousovich-Neto, Raissa Guimarães Ludwig, Victor Corasolla Carregari, Ana Beatriz dos Anjos Souza, Amanda Stephane Cruz dos Passos, Matheus Cavalheiro Martini, Priscilla Paschoal Barbosa, Gabriela Fabiano de Souza, Stéfanie Primon Muraro, Julia Forato, Mariene Ribeiro Amorim, Rafael Elias Marques, Flavio Protasio Veras, Ester Barreto, Tiago Tomazini Gonçalves, Isadora Marques Paiva, Narayana P. B. Fazolini, Carolina Mie Kawagosi Onodera, Ronaldo Bragança Martins Junior, Paulo Henrique Cavalcanti de Araújo, Sabrina Setembre Batah, Rosa Maria Mendes Viana, Danilo Machado de Melo, Alexandre Todorovic Fabro, Eurico Arruda, Fernando Queiroz Cunha, Thiago Mattar Cunha, Marco Antônio M. Pretti, Bradley Joseph Smith, Henrique Marques-Souza, Thiago L. Knittel, Gabriel Palermo Ruiz, Gerson S. Profeta, Tereza Cristina Minto Fontes-Cal, Mariana Boroni, Marco Aurélio Ramirez Vinolo, Alessandro S. Farias, Pedro Manoel M. Moraes-Vieira, Joyce Maria Annichino Bizzacchi, Tambet Teesalu, Felipe David Mendonça Chaim, Everton Cazzo, Elinton Adami Chaim, José Luiz Proença-Módena, Daniel Martins-de-Souza, Mariana Kiomy Osako, Luiz Osório Leiria and Marcelo A. Mori, 29 September 2022, Nature Communications.
DOI: 10.1038/s41467-022-33218-8